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The antioxidant potential and antiproliferative activity of the extracts of<br />seeds (Black Cummins) and honey formulations are to be explored.<br />The gas chromatography-mass spectrum (GC-MS) and Thin Layer Chromatography (TLC) fingerprint o<br />and Honey formulation revealed alkaloid, saponin, volatile oil, flavonoid, glycosides, sugar, and phenolic compound in the extract. GC-MS profiling of the cold extract of<br />seeds and honey formulation shows peaks for eleven fractions of compounds. Using TLC, the phenolic compounds of<br />and honey formulations were separated.<br />The current study discovers the cytotoxic effect of black Cummins seeds and honey formulation on human ovarian cancer (PA-1) cell line as assessed by MTT assay. PA-1 cells were inhibited with the increasing concentration of<br />seeds extract and honey formulation.<br />The study validates the importance of the tested extracts in the treatment of cancer.<br />The study validates the importance of the tested extracts in the treatment of cancer.<br />Tacrolimus is a powerful immunosuppressant and has been widely used in organ transplantation. In order to further explore the role of tacrolimus in liver transplantation, we conducted network pharmacology analysis.<br />GSE100155 was obtained from the GEO database, and the DEGs of liver transplantation were analyzed. [https://www.selleckchem.com/products/ag-1024-tyrphostin.html learn more] The 2D structure of tacrolimus was obtained from the National Library of Medicine, and the pharmacophore model of tacrolimus was predicted using the online tool pharmmapper. Then a network of tacrolimus and target genes was constructed through network pharmacology, and visualization and GO enrichment analysis was performed through Cytoscape. In addition, we also analyzed the correlation between key genes and immune infiltrating cells. The data of GSE84908 was used to verify the changes of key gene expression levels after tacrolimus treatment.<br />The results of network pharmacological analysis showed that tacrolimus had 43 target genes, and the GO enrichment results showed many potential functions. Further analysis found that there were 5 key target genes in DEGs, and these 5 genes were significantly down-regulated in liver transplant patients. Another important finding was that 5 genes were significantly related to some immune infiltrating cells. The results of the GSE84908 data analysis showed that after tacrolimus treatment, the expression of<br />was significantly increased (<br />=0.015).<br />Tacrolimus may inhibit the human immune response by affecting the expression of<br />in liver transplant patients.<br />Tacrolimus may inhibit the human immune response by affecting the expression of DAAM1 in liver transplant patients.Non-small cell lung cancer (NSCLC) is the main reason of cancer linked mortality and around 80% of cases diagnosed in advanced stage. Therefore current study designed to evaluate the deregulation of miRNA-194 and miRNA-192 in different body fluid of Non small cell lung cancer participants. Present study recruited newly diagnosed histopathologically confirmed. It was observed that the 40% NSCLC participants showed elevated miR-194 expression and 60% NSCLC participants showed reduced miR-194 expression in serum sample while in Bronchial wash, only 20% NSCLC participants showed elevated miR-194 expression while 80% showed reduced miR-194 expression (p = 0.003). It was found that the 54% NSCLC participants showed elevated miR-192 expression and 55% NSCLC participants showed reduced miR-192 expression in serum sample while In Bronchial wash sample, only 25% NSCLC participants showed high miR-192 expression while 75% showed low miR-192 expression (P = 0.0004). Expression of miR-194 was significantly associated withvage sample. Decreased poor overall survival was found to be associated with bronchial wash sample with respect to low miR-194 and miR-192 expression while NSCLC participants showed better overall survival with high miR-194 and miR-192 expression. This suggested decreased expression of miR-192 and miR-194 expression could be the potential prognostic marker among NSCLC participants.The recent massive development in the next-generation sequencing platforms and bioinformatics tools including cloud based computing have proven extremely useful in understanding the deeper-level phylogenetic relationships of angiosperms. The present phylotranscriptomic analyses address the poorly known evolutionary relationships of the order Dilleniales to order of the other angiosperms using the minimum evolution method. The analyses revealed the nesting of the representative taxon of Dilleniales in the MPT but distinct from the representative of the order Santalales, Caryophyllales, Asterales, Cornales, Ericales, Lamiales, Saxifragales, Fabales, Malvales, Vitales and Berberidopsidales.Various viruses can infect honey bees, but deformed wing virus (DWV) is considered the most dangerous virus to them and has role in the sudden decline of bee colonies. This virus has different strains; however, there are no available studies to compare the characteristics of these strains utilizing bioinformatics. In this study, 27 strains of deformed wing virus were analyzed based on their sequences and their genetic relationships. Also, some primers were designed and tested to identify their ability to separate DWV strains. The percentages range from 28.99% to 29.63%, 22.28% to 22.78%, 15.73% to 16.28%, and 31.71% to 32.86% for nucleotides A, G, C, and T, respectively in all strains. The numbers of polymorphic sites as well as nucleotide diversity were highly similar in all strains. Statistical analyses generally showed the absence of high variations between sequences. Also, the phylogenetic tree classified strains into three groups. The network between strains of each group was established and discussed based on their geographical locations. Two groups contained strains from USA and Europe while one group contained strains from Asia. Rapid variations and mutations in the sequences of DWV were suggested. Notably, genetic studies on DWV are lacking in some geographical regions. The variations between strains detected in honey bees and other organisms were discussed. Four primers were designed and tested beside two reference primers. One of the designed primers showed the best results in binding with all DWV strains except one.