Plasmonically Improved CRISPRCas13aBased Bioassay for AmplificationFree Detection involving CancerAssociated RNA

Copyright © 2019 International Journal of Preventive Medicine.Background Sedentary lifestyle plays a key role in the emergence of many noncommunicable diseases. AZD9291 concentration Given the importance of physical activity (PA) in population-based studies, the present study was conducted to investigate the pattern of PA and its correlates in the pilot phase of Azar cohort study. Methods In the pilot phase of Azar cohort study, 1236 individuals aged 35-70 years in Khameneh, a city in East Azarbaijan, Iran, were invited to participate in the study. A total of 952 individuals completed the overall and the PA questionnaire, a response rate of 82%. The PA level was evaluated using the classified PA questionnaire based on the equivalent metabolic activities. The general linear model was used to determine the factors affecting PA. Results The overall mean score of PA was 36.54 (standard deviation = 5.3). In multivariate analysis, after adjustment for sociodemographic variables, total PA score was associated with gender (adjusted β = 0.014, confidence interval [CI] 95% = [0.01-0.82]), occupation (adjusted βs ranged over 0.015-0.059, (CI 95% = 0.01-0.079), level of education (adjusted βs ranged over 0.010-0.018, CI 95% = 0.001-0.026). In other word, sex (mean ranged over 35.49-36.81), educational level (mean ranged over 35.01-36.73) and occupation status (mean ranged over 34.62-39.62) were predictors of PA (all P less then 0.05). This variable could also predict 20% of the variance of the PA. Conclusions The current study identifies that gender, occupation and level of education could be factors that influence on PA level in the study population. Copyright © 2019 International Journal of Preventive Medicine.Background To provide a critical reflection from the literature review to describe the current panorama on the importance of physical exercises in the improvement of the quality of life of the aged, besides the use of medications. While aging brings a high cost to health and demographic pyramid changes, so does the disease. As aging is a biological process and many diseases develop at this time of life, the aged are the fastest growing segment of the Brazilian population due to the natural susceptibility of the organism. With this increase, health costs in the country also increase. In everyday practice, medicine has often been characterized as a therapeutic intervention instrument for the first choice, and a consequence, generates significant and significant economic and social damages. Methods Search in databases using analysis descriptors as exercises and medicine for the aged. Results The involvement of the aged in physical exercise programs is an important adjunct to reduce the progressive degeneration associated with natural aging, becoming a powerful instrument to guarantee a healthy, active and independent lifestyle. Conclusions The physical activity was inversely associated with the number of medications, the more physical activity and fewer drug expenses. The need to implement programs for orientation and for the promotion of health through physical activity was also verified. Copyright © 2019 International Journal of Preventive Medicine.Objectives To design a multivalent DNA vaccine encoding the most immunogenic regions of the Leishmania major antigens including TSA (Thiol-specific antioxidant protein), LmSTI1 (Leishmania major stress-inducible protein1), LACK (Leishmania homologue of receptors for activated C Kinase), and KMP11 (kinetoplastid membrane protein-11) on BALB/c mice. Materials and Methods The chimeric construct was generated including the most immunogenic epitopes containing a combination of domains and oligopeptides of the aforementioned genes. The construct was cloned into pcDNA 3.1 plasmid and named "pleish-dom." Following intramuscular injection of mice, the capability of the vector pleish-dom alone and with pIL-12 (expressing murine IL-12) to raise protective cytokines and parasite burden was evaluated in the BALB/c mice as a susceptible animal model against L. major. Results The immunized mice with pleish-dom/pIL-12 showed the highest and the lowest levels of interferon-gamma (IFN-γ) and interleukin-10 (IL-10), as well as the lowest leishmanin skin test (LST) reactions, were found through 8 weeks post-infection. Conclusion Although the obtained DNA vaccine from the immunogenic chimeric protein of L. major antigens was able to induce a high level of IFN-γ, it partially protected mice against L. major. However co-administration with pIL-12 led to shift immune response to Th1 phenotype, granuloma formation, and lowered parasite burden, and consequently distinct protection was found.Objectives Aspiration is a common cause of acute lung injury (ALI), which lacks an effective treatment. Inflammation and oxidative stress play key roles in ALI development. Silymarin is an active extract of Silybum marianum plant seeds (milk thistle). Silymarin has potent anti-inflammatory and antioxidant effects; however its role in aspiration induced ALI has not been investigated. The aim of this study is to investigate the role of silymarin in the treatment of hydrochloric acid (HCl) aspiration induced ALI and explores its mechanisms of action. Materials and Methods The study included three groups of rats Control non-treated group, ALI group (intra-tracheal HCl injected), and silymarin treated ALI group. White blood cells (WBCs) with differential count, oxidative stress parameters, B-cell lymphoma 2 (Bcl-2), transforming growth factor-beta (TGF-β), cyclooxygenase 2 (COX-2), nuclear factor erythroid 2-related factor-2 (Nrf-2), and heme oxygenase-1 (HO-1) were investigated. Lung tissue histopathology and immunohistochemical expression of survivin and proliferating cell nuclear antigen (PCNA) were also examined. Results The results of the study showed that HCL caused histopathological changes in ALI with leukocytopenia and increased oxidative stress biomarkers. It increased TGF-β, up-regulated mRNA expression of COX-2, Nrf-2, and HO-1 and increased survivin and PCNA but decreased Bcl-2. Silymarin ameliorated the histopathological lung injury with further up-regulation of Nrf-2 and HO-1 mRNA and decreased the inflammatory and fibrotic parameters together with up-regulation of the anti-apoptotic and the proliferation parameters. Conclusion The protective effect of silymarin against ALI is mediated by Nrf-2/HO-1 pathway with subsequent antioxidant, anti-inflammatory, antiapoptotic, and proliferating activities.