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The trace metal detection test (TMDT) is an effective and convenient technique to potentially link perpetrators and metallic weapons by comparing morphological information of developed imprints and suspected weapons. However, metallic items without characteristic patterns and incomplete contact with weapons often lead to inadequate morphological features in developed imprints on hands, resulting in difficulty in identifying suspected weapons and a failure to demonstrate potential relationships between perpetrators and weapons. This paper presents a subsequent procedure, after application of the TMDT, for inferring possible weapon-source of a specific imprint. As a proof of concept, all the experiments involved metallic items as an example and were carried out under controlled laboratory conditions. An analytical method was established by selecting elements of interest in developed imprints from seven metallic items (three groups), undertaking quantitative ICP-MS determination of the elements, and comparing the elements in these imprints (inter- and intra-group comparisons) and with those in their source items. Using the established method, possible groups and types of source metallic items could be inferred based on elemental characteristics in imprints, under the premise that no other metal sources exist before or after contacting specified metallic items. This method could be useful for providing investigative clues and evidence of association for developed imprints that lack unique morphological features and for verifying the results of color reactions in the TMDT. For this reason, it can serve as a standard supplementary procedure after the application of the TMDT, which could further strengthen the correlations between perpetrators and weapons even common metallic objects. V.PURPOSE Nowadays, (-)-cannabidiol (CBD) is gaining popularity for the treatment of various problems and can be found easily in many stores in Belgium. However, such product must comply with the law if the total tetrahydrocannabinol (THC) content [(-)-Δ9-tetrahydrocannabinol + (-)-Δ9-tetrahydrocannabinolic acid A (THC-A)] is higher than 0.2%, it is considered as narcotic by the Belgian legislation. In this context, we have developed a method to quantify major cannabinoids (THC, THC-A, CBD, cannabidiolic acid, cannabigerolic acid, cannabigerol and cannabinol) in plant material. METHODS After drying, a liquid-liquid extraction was performed on plant materials, followed by dilutions. Extracts were analyzed by ultra-high-performance liquid chromatography combined with a photodiode array detector. Mobile phases consisted of methanol and 0.1% formic acid in water applied in a 16-minute gradient mode. After validating the method, it was applied to 213 samples seized by the police in CBD shops. RESULTS The method fulfilled the criteria in terms of specificity, calibration curve, precision, trueness and dosing range. Total THC content ranged from 0.14 to 1.17% (median 0.38%) with 110 samples exceeding the Belgian legal threshold of 0.2%. The amounts measured in the samples varied greatly, some were 6 times below the amount labelled on the packaging, others showed a concentration 4 times higher than stated on the package. Same strain also showed concentration differences from shop to shop. CONCLUSION Our method was successfully validated and applied to samples seized in CBD shops. Half of the products exceeded the Belgian legal threshold. THC and CBD concentrations discrepancies showed that products sold in CBD shops are not pharmaceutical grade. Dog attacks today represent a health hazard considering that prevention strategies have not always been successful. The identification of the dog that attacked the victim is necessary, considering the civil or criminal consequences for the animal's owner. An accurate scene analysis must be performed collecting a series of important information. Forensic investigations in dog attacks involve different methods, such as the evaluating of the canine Short Tandem Repeat (STR) typing in saliva traces on wounds or bite mark analysis, however, these techniques cannot always be applied. The effort to find new methods to identify the dog that attacked the victim represents a very interesting field for the forensic community. This study aims to propose an innovative approach, based on the identification of the victim's profile in the dog's mouth, using a buccal swab on the suspected aggressor dog, to find the victim's genetic profile. In addition, a further goal of this study is to determine the persistence time of hexogen DNA in the dog's mouth to define a timeframe for performing this particular technique. For this purpose, ten different dogs were used to aggressively bite a bovine sample (reference sample) to simulate the victim. For each dog two buccal swabs were taken at different time intervals 30', 45', 60', 90', 120', 150', 180' and 240'. The typing of the swabs provided an interpretable profile after 45' while traces of bovine profile were found until 150' after the dog attack simulation. These results could be improved using the human identification kit, which is more sensitive. In the light of this experimental study, the forensic community should consider using this approach in real casework studies with the aim of collecting new data, validating this technique for forensic use. Many types of skin lesions have been described in patients with diabetes, but prolonged prayer-induced hyperkeratosis and callous formation are rare. We describe such lesions in a 62-year-old Muslim patient with uncontrolled glycemia who practiced prolonged, repeated religious prayers in sitting position causing significant pressure over dorsum of both ankles. He developed prayer-induced callous formation on dorsum of both ankles, with more thickening and a healed ulcer on left. Prayer-induced skin lesions should be carefully managed in patients with diabetes, who are more vulnerable for infections and gangrene due to as venous/arterial insufficiency and peripheral neuropathy. BACKGROUND Adequate sleep is crucial for normal functioning. However, most people, including middle-aged adults of reproductive age, show a marked reduction in their sleep duration. Thus, sleep is a major issue that should be addressed in health management. We investigated the effects of short-term sleep restriction on subjective parameters (sleepiness, stress, fatigue) and physiological parameters (cortisol, thyrotropin [TSH], thyroxine [T4], triiodothyronine [T3], C-reactive protein [CRP]) in middle-aged adults, the recovery of these parameters after rest, and the associations between parameters. METHODS A total of 118 healthy adults (59 men, 59 women), aged 35-44 years, and without sleep problems, were enrolled. ACY-775 in vitro Participants underwent a 4-h sleep restriction per day for 3 day at a hospital, and then returned to their daily lives to take four days of rest. A questionnaire and blood test were administered before and after sleep restriction, and after the recovery period, to assess subjective and physiological parameters.