Set up Genome Construction from the Aral Barbel Luciobarbus brachycephalus Utilizing PacBio Sequencing

In this study, the secondary metabolites of a lichen-derived actinomycete strain Amycolatopsis sp. YIM 130687 were investigated intensively by using three different media (4#, 302#, and 312#) for fermentation. A total of 21 compounds were isolated from the fermented extraction of the strain. The structures of all compounds were identified by the examination of HRESIMS and NMR spectra. Compounds 1-3, 5, 6, 21 were only found in the cultivation on 302# medium, while compounds 4, 9-11 were only obtained when the strain was cultured on 312# medium. On the other hand, compounds 7, 8, and 20 were only isolated from the fermentation product on 4# medium. The antimicrobial activity test showed that compound 9 had significant inhibitory effects on bacterial pathogens of Staphylococcus aureus and MRSA with the MICs of 2 μg/ml and fungal pathogens of Botrytis cinerea and Fusarium graminearum with the MICs of 1 μg/ml.Plant growth promoting rhizobacteria (PGPR) are important for agriculture through their activity in stimulating and facilitating plant growth. The rhizobacteria were screened for molecular characterization and followed by their indole acetic acid (IAA) production, phosphate solubility, antibiosis activity. In this study, 162 soil samples were collected from the cocoa rhizosphere to isolate Bacillus subtilis strains using Mössel agar medium with an additional egg yolk and identified by sequencing the ytcP gene. The ability of each strain to form biofilms was obtained in a tube. Indole-3-acetic acid (IAA) production was estimated in Yeast Peptone Dextrose (YPB) broth. Phosphates were solubilized by each strain on Pikovskaya agar medium. The detection of lipopeptide genes using the molecular method has established the possession of isolates by antimicrobial genes. Fifty (50) B. subtilis strains were isolated and identified using the ytcP gene. Ninety percent (90%) of the strains were able to form a biofilm. All isolates produced an IAA. Forty (40 (80%)) of B. subtilis were solubilized phosphate with phosphate solubilizing index (PSI) of 0 to 97.33 ± 0.70%. Of all B. subtilis strains, 45 (90%) have the srfAA gene, 19 (38%) have the fenD gene and 12 (24%) have the ituC gene. Eganelisib B. subtilis strains from cocoa rhizosphere would be beneficial for agricultural production by their PGPR activities.The connection between soil and microbes plays a critical role in soil health and quality and can be elastic with the application of soil amendments and/or crop rotations. Inappropriate management of soil and application of impermissible levels of fertilizers ruptures the overriding connection between the soil and microbes. This is currently evidenced in the degraded soils (i.e., saline soils of India) which are caused by modern agricultural practices. Reclamation of saline soils with a saturated package of practices and conventional breeding methods requires biological intervention. Shortfall of nutrients is one of the chief constraints for plant growth in salt-affected soils. In the present investigation, we have observed an arsenal of fifty halophilic bacteria carrying an absolute requirement of 3% NaCl for solubilizing the insoluble minerals (ZnCO3, ZnO, Mica and tri-calcium phosphate) under in vitro conditions; however, increasing the amount of NaCl over and above resulted in loss of solubilization capacity. Of the isolates solubilizing zinc carbonate and zinc oxide at 3% NaCl concentration, there were 29 isolates; at 10% concentration, 10 isolates were positive for the presence of zinc carbonate. At 3% NaCl concentration, HB-5 showed 23.16 mm zinc carbonate solubilization, HB-20 showed 13.3 mm Zinc oxide solubilization, and HB-7 showed 13.4 mm tri-calcium phosphate solubilization. Mica solubilization was peaked at 6% NaCl and maximum solubilization was observed in HB-27 (18.03 mm). When compared to the zinc carbonate solubilization, zinc oxide solubilization was slow to reach desired levels. Solubilization lasted for up to 9 days and ceased thereafter in all the tests. Eight elite isolates were identified as Bacillus albus, Bacillus safensis, Pseudomonas stutzeri (2), Lysinibacillus sphaericus, Staphylococcus xylosus, and Bacillus cereus (2) based on 16S rRNA analysis.Hepatic veno-occlusive disease (VOD) is a life-threatening complication following hematopoietic stem cell transplant (HSCT). Busulfan has a narrow therapeutic index and its concentration was found to correlate with VOD. Our primary objective was to assess the association between busulfan clearance and VOD in HSCT patients. In this retrospective analysis, we included patients who received their HSCT between 2003 and 2014 and followed at Sultan Qaboos University Hospital. All patients who received dose-targeted busulfan-containing conditioning were included. Target steady-state concentration (Css) was 800-900 ng/ml. VOD was assessed using modified Seattle criteria. The impact of busulfan clearance on VOD was analyzed using univariable logistic regression model. Seventy-three patients were included with a mean age of 15 years. Of those, 47% were transplanted for hematological malignancies and 53% for inherited hemoglobinopathies. Target Css was achieved in 85% of patients. The rate of VOD was 17%. There was no significant impact of busulfan clearance (p = 0.919) or area-under-the-concentration-time-curve (p = 0.275) on VOD. Targeting busulfan Css into narrow therapeutic range may have accounted for the findings. The risk of VOD might be related to other factors such as the genetic background, and more studies are required to investigate these factors.A majority of mesothelioma had the wild-type p53 genotype but was defective of p53 functions primarily due to a genetic defect in INK4A/ARF region. We examined a growth suppressive activity of CP-31398 which was developed to restore the p53 functions irrespective of the genotype in mesothelioma with wild-type or mutated p53. CP-31398 up-regulated p53 levels in cells with wild-type p53 genotype but induced cell growth suppression in a p53-independent manner. In contrasts, nutlin-3a, an MDM2 inhibitor, increased p53 and p21 levels in mesothelioma with the wild-type p53 genotype and produced growth suppressive effects. We investigated a combinatory effect of CP-31398 and nutlin-2a and found the combination produced synergistic growth inhibition in mesothelioma with the wild-type p53 but not with mutated p53. Western blot analysis showed that the combination increased p53 and the phosphorylation levels greater than treatments with the single agent, augmented cleavages of PARP and caspase-3, and decreased phosphorylated FAK levels.