TOP2Bs efforts in order to transcription

To study the peculiarities of ecological relationships of breast cancer (BC) cell lines MCF-7, BT-474and MDA-MD-231under co-culturing conditions.
Three BC cell lines luminal A- MCF-7, luminal B- BT-474and triple-negative- MDA-MD-231were co-cultured pairwise. Immunocytochemistry was used to differentiate the cell lines in the wells. The effect of the cell-free culture medium on the growth rate of the alternate cell line in the pair was also evaluated.
It was shown that when BT-474cells were co-cultured with MCF-7and BT-474cells were co-cultured with MDA-MD-231, two types of ecological interactions could be observed commensalism and amensalism, respectively. While the cells do not interact with each other in contact, the supernatants of single cultures of MCF-7and MDA-MD-231exert the same effect on BT-474as co-cultivation of BT-474with these cells.
The paracrine mechanism of intercellular interaction between different human BC cell lines has been demonstrated. The models used in population ecology can be applicable to identify the types of interaction between cell lines.
The paracrine mechanism of intercellular interaction between different human BC cell lines has been demonstrated. The models used in population ecology can be applicable to identify the types of interaction between cell lines.
Uterine leiomyoma (UL) is the most common benign neoplasm of the uterus. It is still unknown surely what exactly initiates transformation of the uterine myometrial cells into UL.
To study the effect of the TP53gene variants on the risk of development and clinical features of UL.
Case-control study was performed using molecular genetic analyses of variants rs1042522 (119G>C) and rs1625895 (13494G>A) of TP53gene in patients with UL and comparison group of healthy women.
Investigated TP53gene variants were not associated with the risk of UL development. The patients with the 13494GG genotype (rs1625895) had significantly more often subserous UL (р< 0.05). In patients with heterozygous variant of TP53- 13494GA genotype (rs1625895) intramural UL was observed (р< 0.05).
The rs1625895 (13494G>A) variant of TP53gene was associated with UL localization. The identified dependence of the UL localization on the TP53gene variant could be useful for personalized approach to treatment.
A) variant of TP53 gene was associated with UL localization. The identified dependence of the UL localization on the TP53 gene variant could be useful for personalized approach to treatment.
It is known that interactions between tumor and endothelial cells have a significant influence on the growth and metastasis of malignant tumors.
To study the reciprocal effect of Lewis lung carcinoma (LLC) and endothelial cells on the growth rate of each other upon their co-cultivation in vitro and to assess the contribution of such tumor/endothelial cell crosstalk to in vivo LLC growth and metastasis.
Two variants of Lewis lung carcinoma cells, high-metastatic (LLC) and low-metastatic (LLC/R9), and murine aorta endothelial cell line (MAEC) were used. Kinetics of tumor cell growth in vitro and in vivo, electrokinetic properties of tumor cells and their adhesion to endothelial monolayer, and the number of tumor and endothelial viable cells after 1-day contact or non-contact co-cultivation were estimated.
LLC/R9 had significantly higher growth rate in vivo (as opposed to in vitro) than LLC. However, the number and volume of lung metastatic lesions in LLC/R9-bearing mice were 4.5-fold (p < 0.05) and 3 such a subpopulation was absent and 19% of cells had a surface charge < 5 C/m
. The number of LLC cells that adhered to the monolayer of endothelial cells was by 65% (p < 0.05) higher than that of LLC/R9 cells.
Obtained data demonstrated that the tumor/endothelial cell relationships might reflect the features of tumor growth and metastasis of a malignant tumor.
Obtained data demonstrated that the tumor/endothelial cell relationships might reflect the features of tumor growth and metastasis of a malignant tumor.Chronic renal failure is one of the most challenging complications after the completed surgical treatment for renal cell cancer. In 2016, a grading system of tumorous renal involvement was developed, referred to as NCIU nephrometry. However, the systematic parameter to reflect the functional status of the functional renal parenchyma is defined by tumor volume only, with no regard for spatial disposition of the segment(s) where the tumor is located. Our research team decided to improve the NCIU nephrometry system by developing and testing a modified formula for calculation of creatinine clearance, which makes allowance for spatial disposition of tumor within the kidney. NS 105 ic50 We performed numerical computations and analysis of changes in functional status of renal parenchyma depending on coordinate-based spatial location of the tumor in order to augment the existing NCIU nephrometry scale; Matlab, a specialized software package was used as a principal instrument to calculate the number of nephrons and functional renal parenchyma depending on the coordinate-based position of the mass center of the tumor and tumor volume. This model was shown to create a feasible opportunity to increase the percentage of organ-sparing procedures for renal cell cancer and to reduce the incidence/progression of chronic renal failure in these patients.
The expression of the CXCL12chemokine and its receptor CXCR4in the stromal component of the tumor plays an important role in tumor cell migration, proliferation, inhibition of apoptosis and determination of invasive and metastatic potential of malignant neoplasms of various genesis. The significance of CXCL12and CXCR4expression in endometrial tumor cells for cancer progression is not fully understood.
To evaluate the content of CXCL12
-fibroblasts and expression of CXCL12and CXCR4in endometrial cancer cells, depending on the tumor stage.
Surgical material of 45patients with endometrioid carcinoma of the endometrium (ECE) of the stages I-II and III was studied using morphological and immunohistochemical methods.
In ECE of stage I-II CXCR4expression was lower (43.3± 4.2%) while CXCL12expression was higher (33.6± 2.4%) compared with the corresponding indices​​ in ECE of stage III (63.6± 3.5%, 24.5± 1.9%, respectively, p< 0.05). In ECE of stage III, high expression of CXCR4 (> Me) and low CXCL12 (< Me) was observed in 80% of samples; these tumors invaded more than 1/2of the myometrium.